Biofuels Production projects
Robustness to Environmental Heterogeneity – Engineering Strains Optimized for Large-Scale Fermentation - Completed
The team in this project worked to genetically engineer E. coli to efficiently and robustly utilize glucose, arabinose, and xylose for the production of diverse biofuels. They characterized multiple-sugar utilization at a single-cell resolution level. They also constructed a computational model of multiple sugar utilization.
Rao’s group characterized the regulation of C5 and C6 sugar utilization. Their key contribution was determining how E. coli regulates the selective utilization of C5 sugars. They also identified and characterized a number of sugar efflux transporters. They also engineered strains of E. coli to efficiently utilize both C5 and C6 sugars by reprogramming gene expression and sugar transport.
Published in 2010
Regulation of Arabinose and Xylose in Escherichia coli, Tasha Desai and Christopher Rao, Applied and Environmental Microbiology, 76(5): pp. 1524-1532, March 2010.