Biomass Depolymerization projects
Fractionating Recalcitrant Miscanthus by a Two-Stage Treatment under Mild Reaction Conditions - Completed
This project studied the use a two-stage method to fractionate recalcitrant Miscanthus for the production of liquid fuel and value-added products. The team's goals were 1) to delignify Miscanthus and solublize hemicellulose under mild conditions with acidic electrolyzed water or (and) alkaline peroxide; pretreat Miscanthus using alkaline electrolyzed water (ALEW) and to determine generation of inhibitory compounds in pretreated samples; and 3) hydrolyze the pretreated samples using commercial enzyme preparations.
Researchers obtained the highest digestibility with a 4% H2O2 treatment at 50°C for 24 hours for both solid loadings. The feedstock particle sizes also affected the sugar yield; digestibility for a feedstock with particle sizes of 50-80 mesh was about 5% higher than that with 20-80 mesh particles. Feng’s group recorded a high ethanol production from the hydrolysate without detoxification and nutrition fortification. The liquid portion in the first pretreatment was used to extract hemicellulose with an ethanol precipitation method. They recovered up to 99% sugars from hemicelluloses.
Published in 2010
Deconstructing Recalcitrant Miscanthus with Alkaline Peroxide and Electrolyzed Water, Bin Wang, Xiaojuan Wang, Hao Feng, Bioresource Technology, 101(2): pp. 752-760, January 2010.