Biomass Depolymerization programs
Dissecting the Pathway to Hyper-Secretion of Lignocellulolytic Enzymes in Filamentous Fungi
Filamentous fungi degrade most plant material, especially lignin and cellulose. The ultimate goal of this program is to understand the genomic and secretory pathway remodeling that occurs when Neurospora crassa deconstructs plant cell walls/cellulose in order to use rational design to create these industrial strains that secrete high amounts of those enzymes, cellulases and hemicellulases.
The Glass group screened more than 200 mutants and are characterizing about 20 strains with altered enzyme activity or growth on cellulose. They also designed tools to probe the secretory pathway that may yield methods to increase cellulolytic enzyme output. PI's Cate and Glass have also been developing a protein expression system to generate engineered fungal cellulases for industrial use.
Glass’s lab has developed a number of cell biological tools that enable live cell imaging of components of the secretory apparatus during secretion of cellulases/hemicellulases.
Published in 2012
Conserved and Essential Transcription Factors for Cellulase Gene Expression in Ascomycete Fungi, Samuel Coradetti, James Craig, Yi Xiong, Teresa Shock, Chaoguang Tian, N. Louise Glass, Proceedings of the National Academy of Sciences USA, 109(19), pp. 7397-7402, doi: 10.1073/pnas.1200785109, May 8, 2012.
Induction of lignocellulose degrading enzymes in Neurospora crassa by cellodextrins, Znameroski, E.A, S.T. Coradetti, C.M. Roche, J.C. Tsai, A.T. Iavorone, J.H.D. Cate, and N.L. Glass, Proceedings of the National Academy of Sciences USA, 109 (16), pp. 6012-6017, doi: , April 17, 2012.
Deciphering regulatory mechanisms associated with hemicellulose degradation in Neurospora crassa, Sun, J.D., Tian, S. Diamond, and N.L. Glass, Eukaryotic Cell, 11 (4), pp 482-493, doi: 2012.
Unraveling the molecular basis of lilght-modulated cellulase gene expression -- the role of photoreceptors in Neurospora crassa, Schmoll, M., Tian, J. Sun, D. Tisch and N.L. Glass, BMC Genomics 13:127, doi: .
Published in 2011
Identification of the CRE-1 cellulolytic regulon in Neurospora crassa, Jianping Sun and N. Louise Glass, PLoS One, 6: e25654.
Engineered Saccharomyces cerevisiae Capable of Simultaneous Cellobiose and Xylose Fermentation, Suk-Jin Ha, Jonathan Galazka, Soo Rin Kim, Jin-Ho Choi, Xiaomin Yang, Jin-Ho Seo, N. Louise Glass, Jamie H. D. Cate, Yong-Su Jin, Proceedings of the National Academy of Sciences, DOI: 10.1073, January 11, 2011.
Expression and characterization of Neurospora crassa endoglucanase GH5-1, Sun, J., C.M. Philips, C.T. Anderson, W. T. Beeson, M.A. Marletta, and N.L. Glass, Protein Expression and Purification, 11 (4), doe: 10.1016/j.pep.2010.08.016, February 2011.
Published in 2010
Overcoming Glucose Repression in Mixed Sugar Fermentation by Co-Expressing a Cellobiose Transporter and a beta-Glucosidase in Saccharomyces Cerevisiae, Yanglin Li, Jing Du, Jie Sun, John Galazka, Louise Glass, Jamie Cate, Xiang Yang, Huimin Zhao, Molecular Biosystems, (publication ahead of print), September 27, 2010.
Cellodextrin Transport in Yeast for Improved Biofuel Production, Jonathan Galazka, Chaoguang Tian, William Beeson, Bruno Martinez, N. Louise Glass, Jamie Cate, Science, DOI: 10.1126/science1192838, August 25, 2010.