EBI Personnel Directory Savage, David

Biofuels Production

David Savage





Engineering Fluorescent Biofuel Sensors

The high-throughput construction of metabolically engineered organisms is hindered by our inability to rapidly assay the presence of most small molecules. Recently, biosensors fashioned around the green fluorescent protein (GFP) have been developed to sense a number of important chemicals such as calcium and ATP, but little attempt has been made to develop this work in the biofuel space. A fluorescent biosensor of biofuels or biofuel intermediates will have an enormous impact on our ability to construct and evolve production strains with superior yields and rates. This project is engineering GFP-based biosensors of various biofuels (medium and long-chain esters, alcohols, and alkanes) and their intermediates in fatty acid metabolism. A small number of the best biosensors will be optimized and used in a proof of principle experiment to screen for novel mutations to improve the flux and yield of biofuel-producing strains.