EBI Personnel Directory Macdonald, James Irving
James Irving Macdonald
This team is generating cellulase enzymes with enhanced physical properties by attaching polymer stands with low critical solution temperature (LCST) values to specific locations on the cellulase surfaces. Raising the temperature above the LCST threshold value causes the temporary precipitation of the attached cellulases, enabling them to be recovered and added to fresh biomass samples (at temperatures below the LCST) and resume their catalytic activity. This "thermorecycling" concept will improve the practicality and decrease the cost of using these enzymes in cellulose depolymerization, and provide a useful design concept that could be applied to other enzymatic transformations required for feedstock processing.